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Selected array results from HESN and controls were validated by quantitative PCR using LightCycler real time PCR (Roche Diagnostics). And to asses that the gene expression profiles may not have been confounded by sex work, blood samples from sex worker women who have been involved in sex work for one year and were HIV negative (New Negatives) were randomly selected and included as additional controls in qRT-PCR confirmation of microarray data.
Samples were analysed with Quantitect SYBR Green RT PCR assay kit as per the manufacturer’s protocol (Qiagen). Briefly, 100 ng of each sample were ran in duplicate and normalized to 18 sRNA. Relative expression of each gene was determined from a standard curve of pooled cDNA from human peripheral blood mononuclear cells with total RNA extracted in the same way in a 10 series dilution to form the qRT-PCR standards [64]. p -values for significance were analyzed using a student t-test.
The microarray data have been deposited at the Genomic Spatial Event (GSE) database under numbers GSE 30155–30240 and GSE 33580.
We thank the volunteers of the Pumwani sex worker cohort for their invaluable time and contribution to this project. We sincerely acknowledge the support from the clinic staff of the University of Nairobi/University of Manitoba Collaborative Program for sample and data collection. Our gratitude to Mark Jordan and Brenda Oosterveen of Agriculture Canada for validating our microarray assays, Leslie Slaney and Ian Maclean of University of Manitoba for logistical support and Rose Odera and Salome Ngamau of Kenya Medical Research Institute for secretarial services.
Author Contributions.
Conceived and designed the experiments: EMS BTB FAP. Performed the experiments: EMS WA NK. Analyzed the data: EMS GB BL. Contributed reagents/materials/analysis tools: ML PM RS JK CW KF. Wrote the paper: EMS.
Early Christmas as Airtel Uganda Staff Give Back.
For the third consecutive year, Airtel Uganda has embarked on an initiative to share the joy of Christmas with individuals, families, organizations and communities through their staff-driven initiative dubbed ‘12 Days of Christmas’.
Named after a popular Christmas song that breaks down a series of grand gifts given on each of the twelve days of Christmas, this initiative aims at rewarding individuals and organizations that have made a remarkable difference in the lives of their community members. This initiative also gives Airtel Uganda staff members an opportunity to assist individuals and communities in need.
Airtel Uganda staff members celebrate early Christmas with Salama School of the Blind.
The telco’s staff begun by spreading Christmas cheer to the African Children of God’s Image (ACOGI) located in Katanga and Salama School for the blind in Mukono. The staff members handed over foodstuffs such as posho, rice and sugar; scholastic materials such as books and pens as well as some musical instruments to ACOGI and a Perkins Brailler (a gadget used by the visually impaired to write and read), sauce pans, cups, school bags, bottles and sodas to Salama school for the blind. In the spirit of Christmas, the staff members cut cake with the children.
Airtel Uganda staff led by Marketing Director Mr. Indrajeet Kumar carry foodstuffs during the visit to African Children of God’s Image in Katanga.
Commenting about the initiative, Mr. V.G. Somasekhar, the Airtel Uganda Managing Director, applauded the staff members for their commitment to give back to society.
“Through this initiative, Airtel Uganda staff members have managed to change the lives of hundreds of Ugandans in different communities across the country in such a short time,” he commented.
Airtel Uganda staff members spread the joy of Christmas to children in Katanga, Wandegeya.
“We believe it is our responsibility to give back to Ugandans in the communities in which we operate by not only providing reliable telecommunications products and services, but also offering them assistance to enable them lead more meaningful and productive lives,” he added.
As part of this year’s initiative, staff members will visit the Children’s Cancer Ward Mulago, SOTEDA Diabetics Organization in Gulu, Kankobe Children’s Center in Masaka, Mugalula Community in Wakiso, Boda Boda Initiative in Kamuli, Busebewe Primary School in Luwero, Prince of Peace Nursery and Primary in Kobwin, Kyamulinga Public School in Mityana, Ibanda Babies Home and Special Children Special People in Ggaba.
The Shia practice of the forbidden temporary marriage.
All praise is due to Allah, Whom we ask for help and forgiveness. We seek the protection of Allah from the sins of ourselves and our deeds. No one can misguide whom He guides, and whom He does not guide, will never find a guide.
I testify that there is no God but Allah, the One with no partner, and that Muhammad (s.a.w) is His slave and Messenger.
In the days before the battle of khaibar the Prophet permitted the use of temporary marriage.
" Narrated Salama bin Al-Akwa’ (r.a): ‘In the year of Autas, Allahs Messenger permitted a temporary marriage for three nights, but he prohibited it afterwards . "

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